The quiet dangers of civilized rage: surveying the punitive aftermath of England's 2011 riots

This article considers the state’s punitive response to the civil unrest that swept across England in August 2011. Surveying measures taken by police, courts, and politicians—including lengthy prison sentences, violent police raids, increased surveillance, and proposals for new benefit sanctions—the article highlights the targeting of disaffected young people, impoverished neighborhoods, and racialized communities. Considering these measures alongside the accompanying political rhetoric, the article argues that the state’s actions worked to depoliticize the riots and generate a narrative of blame that worked to rationalize the state’s own vindictive violence

Reflections on Disability, Justice and Abolition

This piece offers reflections on Liat Ben-Moshe’s recent book Decarcerating Disability: Deinstitutionalization and Prison Abolition and Linda Steele’s recent book Disability, Criminal Justice and Law: Reconsidering Court Diversion and their contributions to abolitionist work, disability justice and decarceration

Queer desires and critical pedagogies in higher education: reflections on the transformative potential of non-normative learning desires in the classroom

This article considers what a queer approach might offer in addressing some of the challenges of higher education in the contemporary neoliberal landscape. Despite a rich literature on queer issues in the classroom, most of the existing scholarship has focused on engaging queer students, being a queer teacher, or teaching queer content in the curriculum. Very little work has focused on what it means to take a queer approach to pedagogic techniques or how such an approach might impact educational practices more broadly. We ask: What does it mean in theory and practice to “queer” our teaching methods? What role can queer pedagogic practices play in contesting the marketization of higher education and the shift towards more instrumentalist and consumer-based modes of learning? We argue that a queer approach to pedagogy, which explicitly seeks to open up spaces for non-normative educational desires to emerge, potentially offers fruitful strategies for fostering critical and transformative learning

Reflections on disability, justice and abolition

This piece offers reflections on Liat Ben-Moshe’s recent book Decarcerating Disability: Deinstitutionalization and Prison Abolition and Linda Steele’s recent book Disability, Criminal Justice and Law: Reconsidering Court Diversion and their contributions to abolitionist work, disability justice and decarceration

Unpalatable dissent and the political distribution of solidarity

This article questions the conditions in which solidarity is given or withheld in response to expressions of dissent. Drawing on the August 2011 riots in England as an example, the article reflects on why some forms of dissent attract support whereas others do not. The author argues that ‘unpalatable’ forms of dissent, particularly those enacted by groups already constructed as deviant or suspect, are often figured as least deserving of support, even though their actions may arise from the highest needs. The article then considers how these patterns can occur in response to more everyday articulations of dissent, such as those expressed by disenfranchised university students. The article suggests a rethinking of the politics of dissent and the distribution of solidarity in order to be more attentive to broader patterns of power and dispossession

Directed evolution of Thermus aquaticus DNA polymerase by compartmentalised self-replication

The thermophilic enzyme, Thermus aquaticus (Taq) DNA polymerase, is an essential tool in molecular biology because of its ability to synthesis DNA in vitro and its inherent thermal stability. Taq DNA polymerase is widely used in the polymerase chain reaction (PCR), an essential technique in a broad range of different fields from academic research to clinical diagnostics. The use of PCR-based tests in diagnostic testing is ever increasing; however, many of the samples being tested contain substances that inhibit PCR and prevent target amplification. Many attempts have been made to engineer polymerases not only to increase resistance to overcome the problem of inhibition, but also to enhance other characteristics such as fidelity, processivity and thermostability. Heparin, found in blood samples, and phytate, found in faecal samples, are two examples from a number of known PCR inhibitors. The mode of action of most PCR inhibitors is not well understood, but inhibition is thought to occur by enzyme binding or through the chelation of Mg2+ ions essential for PCR. In this project, a system of directed evolution by compartmentalised self-replication (CSR) was established and successfully employed to screen a mutant library for Taq DNA polymerase variants with enhanced resistance to the inhibitors heparin and phytate. CSR is a recently-established high-throughput method for the creation of novel polymerases, based on a feedback loop whereby polymerase variants replicate their own encoding gene. A mutant library of 106 variants was produced by random mutagenesis error-prone PCR, in which only the polymerase domain of Taq was mutagenised. Firstly, the CSR system was established and tested by performing a screen in the presence of heparin to select for heparin-resistant variants. Characterisation of selected variants revealed that a single round of CSR had produced a Taq variant (P550S, T588S) with a 4-fold increase in heparin resistance. The IC50 was increased from 0.012U/ml heparin to 0.050U/ml heparin. The study with heparin was followed by a phytate screen, in which two rounds of CSR were performed with an initial round of error-prone PCR followed by re-diversification (recombination) of the mutant library using the staggered extension process (StEP). The two rounds of CSR yielded a Taq variant with a 2-fold increase in phytate-resistance compared to the wild-type, with IC50 increased from 360μM phytate to 700μM phytate. The best phytate mutant (P685S, M761V, A814T) was further characterised and it was found that the catalytic activity, thermostability and fidelity of the mutant were comparable to the wildtype enzyme. The position of resistance-conferring mutations of the novel Taq variants evolved in this study provided some evidence for the inhibitors’ predicted modes of action in the case 2 of both phytate and heparin. As phytate’s mode of action is poorly understood, further investigations were performed to elucidate its role in PCR inhibition. A thorough investigation into the importance of relative phytate and Mg2+ levels on PCR was conducted and revealed for the first time convincing evidence that the primary mode of phytatemediated PCR inhibition is by chelation. Further work led to the successful crystallisation of Taq in the presence of phytate, although subsequent X-ray diffraction data to 2.5Å did not reveal phytate bound within the enzyme structure. Site-directed mutagenesis studies were used to probe cross-over between heparin and phytate-conferring mutations. Thus, in addition to providing valuable information for novel Taq variants with a potential application in fecal-based PCR diagnostic tests, this project has begun to provide insight into the fundamental aspects of the mode of action of phytate as a polymerase and PCR inhibitor.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

The false promise of hate crime laws

A blog piece which explains how hate crime laws work and argues that longer sentences and widening criminalisation does not increase community safety

The marketisation of prison alternatives

A short article on why market logics curtail possibilities for genuine alternatives to prison